Curcuma longa rhizome extracts were evaluated for antibacterial activity against pathogenic strains of Gram-positive (Staphylococcus aureus, Staphylococcus epidermidis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium) bacteria. Essential oil was found to be most active and its activity was compared to standard antibiotics gentamycin, ampicillin, doxycycline and erythromycin in these strains. Only the clinical isolate of S. aureus showed more sensitivity towards essential oil fraction than the standard strain. The use of essential oil from turmeric as a potential antiseptic in prevention and treatment of antibacterial infections has been suggested.