The present study aimed to isolate and characterize bioactive components from the polar fractions of CL extracts using bioassay-guided fractionation and to evaluate the immunomodulatory activities of such components using human peripheral blood mononuclear cells (PBMC). The chemical profile of the bioactive component would be characterized by GC–MS and gel permeation chromatography. The cell proliferation responses, cytokine production as well as the distribution of T cell subsets of PBMC would be evaluated.