In Vitro Activity of Hyssopus Officinalis, Tussilage Farfara, Carum Copticum Extracts Against Leishmania Major in Iran

Leishmania is transmitted by sand flies that ingest the parasite in the amastigote phase resident within macrophages, then inoculate the promastigote phase into body hosts. The present study was conducted to evaluate the in vitro effects of alcoholic extract of Plants on L.major. The extract of aerial parts of plants were obtained by maceration. The in vitro experiments were performed on promastigotes to assess antileishmanial activity of the extracts using glucantime as reference. The extract of plants and glucantime solutions for biological testing were prepared in PBS at 0.05-0.1-0.2-0.4 μg/mL and 1μg/mL, respectively. All experiments were repeated at least three times in duplicate. For the extract of plants and glucantime, the concentration-response curve was plotted, from whichIC50 values were determined also No and MTT assay were done. The different concentrations resulted in different optical densities or inhibitory percentages(P<0.05) so that extract of plants were effective against L. major in vitro. NO production the extract of plants showed significant in vitro antileishmanial activities.The Findings of this study indicate that these plants are effective against L.major in vitro.