Several research publications are published on the medicinal plant Hyssopus officinalis L. But, the researchers find quite a difficulty to study all the publications in a short while. The present review was designed to compile and summarize all the published works on the medicinal plant H. officinalis L. traditionally used in several systems of medicine (1885-2018). It showed that the medicinal plant H. officinalis L. belonging to family Lamiaceae, is a very important culinary, medicinal and perennial plant wildly cultivated in Asia, Europe and America. It possesses numerous phytoconstituents including quercetin-7-O-β-D-apiofuranosyl-(1→2)-β-D-xylopyranoside and quercetin-7-O-β-Dapiofuranosyl-(1→2)-β-D-xylopyranoside-3’-O-β-D-glucopyranoside and possesses antioxidant, anticonvulsant, antifungal, antimicrobial, antihemolytic, antiulcer, antispasmodic and several other pharmacological activities. Its essential oil is widely being used in cosmetic, food and pharmaceutical industries worldwide. Its oil is used as anherbal medicine and very precious food additive. It is one main ingredient of the official formulation of Chartreuse. Za’atar is its well-known herbal formulation. It is a quite significant medicinal plant which can be utilized for the treatment of several diseases such as microbial infection, epilepsy, ulcer, and spasm.