Biochemical, Enzymatic, and Immunological Study on Antimutagenic Achillea millefolium Methanolic Extract in vivo

Traditionally medicinal plants are used for its potential chemotherapeutic action and for safety upon the continued use of these plants. Achillea millefolium (AM) is an ancient herb which is used to treat wounds, headaches, disorder in gastrointestinal and hepatobiliary, pain, and inflammation. This study was aimed to investigate the biochemical parameter of antimutagenic methanolic extract for AM in vivo. Plant extract together with MTX decreased the damage caused by the drug on the concentrations of total cholesterol and triglycerides (160.3±2.26 and 108.7±2.71), significant increase in total protein concentration (8.51±0.17, 9.46±0.19 and 10.8±2.71 g\dL) for 100 and 200 mg\kg was observed. Also plant extracts counteract the damage caused by drug through the reduction for both creatinin and albumin concentrations to 1.57±0.03 mg\dL and 4.56±0.09 g\dL, respectively. On enzymatic level, the results of interactions indicated the ability of plant to modulating harmful effects of the drug (57.1±1.3, 130±3.1 and 111.1±2.1 U\L) for GOT,GPT and ALP, respectively. On the other hand, the plant possesses the ability to enhance the concentrations of all immunoglobulin(IgM, IgA and IgG) in the two doses tested. Therefore, the results of this study indicate the antimutagenic potential of AM extract and encouraging its consumption with MTX as one of the promising therapies