The auricular edema is achieved by applying 12-0- Tetradecanoil Forbol-13 Acetate (TPA), one of the components responsible for the irritating action of croton oil, into the auditory pavilion of the mouse. The inflammatory reaction consists of erythema , edema and infiltration by polymorphonuclear leukocytes. As such, eicosanoid -type mediators are freed, inducing degranulation of the mast cell. This technique thus allows the evaluation of the inhibiting substances of the biosynthesis of prostaglandins and leukotrienes.

Takuna will be used in humans because of the vital importance of carrying out these first-step tests. They will not only guarantee the quality of the product, but will also establish that there are no adverse side effects in humans who take the product. As discussed in numerous international works, the study of acute toxicity is indispensable, and guarantees (within the margin of error associated with the technique) that the potential for toxicity from the compounds that will be ingested or that may enter into the system accidentally will be learned.